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[交流]
復乳法制備PLGA微球
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看到一篇文獻制備PLGA微球包裹蛋白酶和疏水小分子藥物的,用的是W/O/W的方法,具體步驟如下: Firstly, we prepared the oil phase with 2 ml CH2Cl2 containing 5 mg/ml PLGA. 0.4 ml 1% w/v PVA solution containing 2.5 mg/ml Cat was used as water phase. To obtain the first emulsion solution, the CH2Cl2 solution was slowly added to the PVA solution by sonication under ice- DMSO (5 mg/ml) was drowsed in 10 ml 2% w/v PVA solution for the second emulsion process. Afterwards, the mixture was homogenized using Selecta Sonopuls in ice bath to form w/o/w PLGAR837@Cat nanoparticles. 請問怎么感覺這個方法不對呢,像是O/W/W呢,想?yún)⒖妓鰧嶒,不知道步驟有沒有錯。 |
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