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[求助]
環(huán)丙沙星
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環(huán)丙沙星用什么溶解進HPLC,我已經(jīng)試了甲醇,二氯,DMSO,DMF,乙腈,乙酸,好像都溶解不了,按照藥典里的7%磷酸溶液也融不了,為2mg,在1ml及以上溶劑。 @lifeliuyan 發(fā)自小木蟲Android客戶端 |
至尊木蟲 (知名作家)
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你是按照usp嗎? Solution A: 0.025 M phosphoric acid. Adjust with triethylamine to a pH of 3.0 ± 0.1. Mobile phase: Acetonitrile and Solution A (13:87) Standard solution: Transfer 12.5 mg of USP Ciprofloxacin RS to a 25-mL volumetric flask. Add 0.1 mL of 7% phosphoric acid, and dilute with Mobile phase to volume. System suitability stock solution: 0.025 mg/mL of USP Ciprofloxacin Ethylenediamine Analog RS in Mobile phase System suitability solution: Transfer 1.0 mL of the System suitability stock solution to a 10-mL volumetric flask, and dilute with the Standard solution to volume. Sample solution: Transfer 25 mg of Ciprofloxacin to a 50-mL volumetric flask. Add 0.2 mL of 7% phosphoric acid, and dilute with Mobile phase to volume. 發(fā)自小木蟲IOS客戶端 |
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