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[求助]
關(guān)于鎳柱重生 已有1人參與
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我想將我做蛋白純化的鎳柱重生,請問方法是什么? @hc-material @gyesang @gyesang 發(fā)自小木蟲Android客戶端 |
版主 (著名寫手)
己所不欲,勿施于人
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步驟如下: Wash column with 3× volume of the resin of Water. Drain completely. Wash column with 3× volume of the resin of 100 mM of EDTA at pH 8.0. Drain completely. Wash column with 5× volume of the resin of Water. Drain completely. Wash column with 1× volume of the resin of 0.2 M NaOH. Drain completely. Wash column with 5× volume of the resin of Water. Drain completely. Wash column with 2× volume of the resin of 0.1 M NiSO4 or NiCl2. Drain completely. Wash column with 5× volume of the resin of Water. Drain completely. Store the resin with 20% ethanol. |

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