 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 3 | 1/1 | 返回列表 |
| 查看: 705 | 回復(fù): 2 | |||
| 【懸賞金幣】回答本帖問題，作者M(jìn)r ironic將贈送您 5 個(gè)金幣 | |||
Mr ironic新蟲 (初入文壇)
|
[求助]
sod pod cat 聯(lián)測的方法 已有1人參與
|
||
|
sod pod cat 聯(lián)測的方法 發(fā)自小木蟲Android客戶端 |
回復(fù)此樓» 猜你喜歡
085701環(huán)境工程專碩求調(diào)劑
已經(jīng)有6人回復(fù)
-
0817化學(xué)工程319求調(diào)劑
已經(jīng)有10人回復(fù)
-
伙伴們，祝我生日快樂吧
已經(jīng)有5人回復(fù)
-
274求調(diào)劑0856材料化工
已經(jīng)有10人回復(fù)
-
337一志愿華南理工0805材料求調(diào)劑
已經(jīng)有6人回復(fù)
-
（081700）化學(xué)工程與技術(shù)-298分求調(diào)劑
已經(jīng)有7人回復(fù)
-
材料與化工304求B區(qū)調(diào)劑
已經(jīng)有5人回復(fù)
-
NSFC申報(bào)書里申請人簡歷中代表性論著還需要在申報(bào)書最后的附件里面再上傳一遍嗎
已經(jīng)有9人回復(fù)
-
一志愿北京化工大學(xué)材料與化工296分求調(diào)劑
已經(jīng)有17人回復(fù)
-
0703化學(xué)一志愿211 總分320求調(diào)劑
已經(jīng)有3人回復(fù)
singchenyao
金蟲 (正式寫手)
- 應(yīng)助: 13 (小學(xué)生)
- 金幣: 3468.2
- 散金: 82
- 紅花: 5
- 帖子: 477
- 在線: 287.2小時(shí)
- 蟲號: 1159599
- 注冊: 2010-12-01
- 性別: GG
- 專業(yè): 農(nóng)學(xué)基礎(chǔ)
【答案】應(yīng)助回帖
|
我這里用傳統(tǒng)的方法測定。我前段時(shí)間測的。 Superoxide dismutase (SOD) activity was analyzed using the nitroblue-tetrazolium (NBT) photoreduction method at 560 nm. One unit of SOD activity was defined as the amount of the enzyme that inhibited NBT reduction by 50% and was expressed as units g−1 fresh weight. For the catalase (CAT) activity assay, the reaction mixture contained 2 ml of 50 mM potassium phosphate buffer (pH 7.0), 1 ml of 15 mM H2O2 and 150 µl of the enzyme extract. The reaction was initiated with the addition of hydrogen peroxide, and the decrease in absorbance was measured at 240 nm for 2 min. CAT activity was expressed as the change in absorbance per min per fresh weight (ΔOD240 min-1 g-1 FW). For the determination of peroxidase (POD) activity, 50 μl of crude extract was added to 3 ml of substrate mixture containing 0.1 M potassium phosphate buffer (pH 6), 15 mM guaiacol and 7.5 mM of 30% H2O2. The formation of the oxidized tetraguaiacol polymer was monitored at 470 nm for 3 min. |
2樓2020-10-12 15:40:31
singchenyao
金蟲 (正式寫手)
- 應(yīng)助: 13 (小學(xué)生)
- 金幣: 3468.2
- 散金: 82
- 紅花: 5
- 帖子: 477
- 在線: 287.2小時(shí)
- 蟲號: 1159599
- 注冊: 2010-12-01
- 性別: GG
- 專業(yè): 農(nóng)學(xué)基礎(chǔ)
3樓2020-10-12 15:58:27
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 Mr ironic 的主題更新
| 3 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 0817化學(xué)工程319求調(diào)劑 +8 | lv945 2026-03-08 | 10/500 |
|
|---|---|---|---|---|
|
[考研] 材料工程,326分，求調(diào)劑 +5 | KRSLSR 2026-03-10 | 5/250 |
|
|
|
[考博] 讀博申請 +5 | 感dd 2026-03-10 | 7/350 |
|
|
|
[考研] 化工0817調(diào)劑 +8 | 燦若星晨 2026-03-10 | 8/400 |
|
|
|
[考研] 332材料求調(diào)劑 +6 | zjy101327 2026-03-05 | 8/400 |
|
|
|
[考研] 材料與化工求調(diào)劑 +11 | 與冬清寧 2026-03-07 | 14/700 |
|
|
|
[考研] 278求調(diào)劑 +8 | Gale1314 2026-03-06 | 8/400 |
|
|
|
[考博] 找博導(dǎo) +4 | 小呆呆熊 2026-03-07 | 4/200 |
|
|
|
[考研] 考研一志愿長安大學(xué)材料與化工309分請求調(diào)劑 +6 | dtdxzxx 2026-03-06 | 8/400 |
|
|
|
[基金申請] 面上項(xiàng)目還需要AI說明嗎？ +3 | liyundong 2026-03-08 | 3/150 |
|
|
|
[考研]
 295復(fù)試調(diào)劑
+4
|
簡木ChuFront 2026-03-09 | 4/200 |
|
|
|
[考研] 一志愿山東大學(xué)，總分327，英語二79，有論文，有競賽，已過四六級 +3 | 木木目目1 2026-03-09 | 3/150 |
|
|
|
[考研] 0703求調(diào)劑 +10 | c297914 2026-03-06 | 11/550 |
|
|
|
[考研] 一志愿211 材料與化工 280求調(diào)劑 +3 | Sanity蔣 2026-03-08 | 3/150 |
|
|
|
[考研] 269求調(diào)劑 +3 | 朔朔話 2026-03-08 | 4/200 |
|
|
|
[考研] 347求調(diào)劑 +4 | 浮云滿足 2026-03-07 | 4/200 |
|
|
|
[考研] 一志愿中國石油大學(xué)（華東） 本科齊魯工業(yè)大學(xué) 求調(diào)劑 +3 | snw石 2026-03-07 | 3/150 |
|
|
|
[考研] 材料專碩323求調(diào)劑 +5 | 李白26 2026-03-07 | 5/250 |
|
|
|
[考研] 材料與化工304求調(diào)劑 +7 | 邱gl 2026-03-05 | 10/500 |
|
|
|
[考研] 求調(diào)劑，學(xué)校研究所都可以，材料與化工267分 +6 | wmx1 2026-03-05 | 6/300 |
|