| 5 | 1/1 | 返回列表 |
| 查看: 1328 | 回復(fù): 4 | ||
秦紫蓉新蟲 (初入文壇)
|
[求助]
DNA序列上傳NCBI問題求解
|
|
在NCBI上傳動(dòng)物COI序列后收到NCBI如下郵件,望有經(jīng)驗(yàn)前輩賜教,該如何解決。本人比較外行,DNA提取擴(kuò)增后都是由測序公司測序拼接的,對基因序列中存在的問題也不太清楚。NCBI郵件如下: We have received the following 57 sequence submissions from you: SUB7027792 : (57) Some or all of the protein coding sequences contain reading frame shifts (insertions/deletions based on BLAST similarity search results and/or an alignment), and/or have translations that show little or no similarity to other proteins in the database. This generally indicates problems such as: - errors in the nucleotide sequence - sequences have not been trimmed of low quality - problems with feature annotation which may be due to: translation in the wrong reading frame or on the wrong strand translation does not have the correct exon spans translation includes noncoding sequence, such as introns or UTR's Provide a corrected submission file through the Submission Portal: |
版主 (著名寫手)

新蟲 (小有名氣)
新蟲 (初入文壇)
| 5 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 一志愿武漢理工大學(xué)-085602-總分296分-求調(diào)劑 +4 | 紫川葡柚 2026-03-04 | 4/200 |
|
|---|---|---|---|---|
|
[考研] 0856材料與化工求調(diào)劑! +3 | 化工考生111 2026-03-04 | 7/350 |
|
|
[考研] 085601 材料工程 320 +6 | 和樂瑤 2026-03-03 | 6/300 |
|
|
[考研] 266求調(diào)劑 +6 | 哇塞王帥 2026-03-03 | 6/300 |
|
|
[考研] 求調(diào)劑 +3 | 泡了個(gè)椒 2026-03-04 | 3/150 |
|
|
[考研]
085600 英一數(shù)二272求調(diào)劑
5+6
|
vida_a 2026-03-01 | 47/2350 |
|
|
[考研] 一志愿西交大材料學(xué)碩 346 求調(diào)劑 +3 | zju51 2026-03-04 | 3/150 |
|
|
[考研] 289求調(diào)劑 +8 | yang婷 2026-03-02 | 10/500 |
|
|
[考研] 291求調(diào)劑 +3 | 咕嚕咕嚕123123 2026-03-02 | 4/200 |
|
|
[考研] 298求調(diào)劑一志愿中海洋 +3 | lour. 2026-03-03 | 3/150 |
|
|
[考研] 江蘇省農(nóng)科院招調(diào)劑1名 +5 | Qwertyuop 2026-03-01 | 5/250 |
|
|
[考研] 化工專碩348,一志愿985求調(diào)劑 +8 | 弗格個(gè) 2026-02-28 | 11/550 |
|
|
[考研] 268求調(diào)劑 +6 | 好運(yùn)連綿不絕 2026-03-02 | 6/300 |
|
|
[考研] 化學(xué)0703求調(diào)劑 學(xué)碩 理/工科均可 總分279 +3 | 1一11 2026-03-03 | 5/250 |
|
|
[考研] 291求調(diào)劑 +3 | MuoLuo1312 2026-03-02 | 6/300 |
|
|
[考研] 清華大學(xué) 材料與化工 353分求調(diào)劑 +5 | awaystay 2026-03-02 | 6/300 |
|
|
[考研] 【2026 碩士調(diào)劑】課題組 招收調(diào)劑生 +3 | 考研版棒棒 2026-03-02 | 5/250 |
|
|
[考研] 化學(xué),材料,環(huán)境類求調(diào)劑 +7 | 考研版棒棒 2026-03-02 | 7/350 |
|
|
[考博] 誠招農(nóng)業(yè)博士 +3 | 心欣向榮 2026-02-28 | 3/150 |
|
|
[考研] 313求調(diào)劑 +3 | 水流年lc 2026-02-28 | 3/150 |
|