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小雞蛋~新蟲 (小有名氣)
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[求助]
有機溶劑萃取
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煩請各位大神幫忙看下圖片里這段文獻。如果能幫忙解釋下操作步驟就更好了。新手上路,感激不盡! 發(fā)自小木蟲Android客戶端 |
捐助貴賓 (文學(xué)泰斗)
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Cell suspension was centrifuged at 4000rpm for 20 min and 10 ml of cell-free supernatant harvested during the stationary growth phase was extracted using the procesdure described previously [30] 細胞懸液以4000rpm離心20 min,然后利用前面描述的工藝提取靜止生長階段收獲的10 ml無細胞上清液[30]。 In brief, 10 ml of cell-free supernatant solution was extracted three times with an equal amount of chloroform. 簡而言之,取10ml無細胞上清液,用等量氯仿三次萃取。 The combined orgainc phases were washed with 2 mL of water and taken to dryness in the oven kept at 20. 將混合后的有機相用2毫升水沖洗干凈,放入烤箱干燥,溫度保持在20℃。 The residue was redissolved in 2 mL of acetonitrile. 殘渣在2毫升乙腈中重新溶解。 This culture solution was stored at freezing temperature until further use. 該培養(yǎng)液冷凍保存,待進一步使用。 |
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