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[求助]
1、4 vol of 0.1 M NaCl什么意思2、含0.6 M NaCl的磷酸鹽緩沖液 (50mM, pH6.0)配置
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題目:Then, the myofibrillar pellets were washed three more times with 4 volof 0.1 M NaCl under the same conditions as mentioned above except that in the last wash,the myofibrillar suspension was filtered through a 40 mesh sifter to remove connective tissue and its pH was adjusted to 6.25 (simulating the pH conditions in processed meats) with 0.1 M HCl prior to centrifugation. 然后,用4 vol of 0.1 M NaCl對肌原纖維微顆粒進行三次洗滌,除了最后那次洗滌外,,用40目篩子過濾肌纖維懸浮液去除結締組織,離心前用0.1 M HCl將其pH值調整為6.25(模擬加工肉類的pH值)。 問題:里面的4 vol of 0.1 M NaCl是什么意思? 題目:Finally, the pellets were suspended in PBS (50 mM[], pH6.0) containing 0.6 M NaCl and the concentration of protein was determined by Biuret with BSA as the standard. 最后,將肌原纖維顆粒懸浮于含0.6 M NaCl的磷酸鹽緩沖液 (50mM, pH6.0)中,以BSA為標準,用雙縮脲法測定蛋白濃度。 問題:含0.6 M NaCl的磷酸鹽緩沖液 (50mM, pH6.0),配置是不是用0.6mol/l的NaCl作為溶劑配置磷酸鹽緩沖液,里面的ph值,是用NaOH調的嗎? |
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